Clubs found out their third-round opponents during the draw, with teams involved in European competitions entering the EFL at this stage.
The EFL Cup heats up with the addition of top-flight clubs Manchester United, Arsenal, Liverpool, Tottenham, Chelsea, Wolves and 2018-19 winners Manchester City in the third round.
The 16 matches commence on Monday September 23.
Full draw results
Wolves vs Reading FC
Oxford United vs West Ham United
Watford vs Swansea
Brighton vs Aston Villa
Sheffield United vs Sunderland
Colchester United vs Tottenham Hotspur
Portsmouth vs Southampthon
Burton Albion vs Bournemouth
Preston North End FC vs Man City
MK Dons vs Liverpool
Man Utd vs Rochdale AFC
Luton FC vs Leicester City
Chelsea vs Macclesfield Town/Grimsby Town
Sheffield Wednesday vs Everton
Arsenal vs Forest
Crowley vs Stoke City